It is the gold standard to determine cell reproductive death after treatment with ionizing radiation. AvailabilityĬlonogenic assay or colony formation assay (CFA) is an in vitro cell survival assay based on the ability of single cells to grow into colonies.
Colony survival sale software#
As open source software interlaboratory code sharing between users is facilitated. The dispersion parameter for goodness of fit and different plot functions in CFAssay help to evaluate experimental data quality. However the dispersion parameter of the quasi-likelihood method is much more sensitive for statistical variation in the data than the multiple R 2 coefficient of determination from the least squares method. Resultsįor the two presented examples estimated parameters do not differ much between maximum-likelihood and least squares. A test for comparision of cell survival curves and an ANOVA test for experimental two-way designs are provided. MethodsĬFAssay offers maximum likelihood and related methods by default and the least squares or weighted least squares method can be optionally chosen. For easy code exchange and methodological standardisation among collaborating laboratories a software package CFAssay for R (R Core Team, R: A Language and Environment for Statistical Computing, 2014) was established to perform thorough statistical analysis of linear-quadratic cell survival curves after treatment with ionizing radiation and of two-way designs of experiments with chemical treatments only. Associated linear-quadratic cell survival curves can be calculated with different methods. All other trademarks, logos, and copyrights are property of their respective owners.Colony formation assay is the gold standard to determine cell reproductive death after treatment with ionizing radiation, applied for different cell lines or in combination with other treatment modalities. © 2020 Paradox Interactive AB, SURVIVING THE AFTERMATH, and PARADOX INTERACTIVE are trademarks and/or registered trademarks of Paradox Interactive AB in Europe, the U.S., and other countries. Graphics: Nvidia® GeForce™ GTX 760 or AMD® Radeon™ R9 380 Processor: Intel® iCore™ i5-3570K or AMD® Ryzen™ 3 2200G Graphics: Nvidia® GeForce™ GTX 580 or AMD® Radeon™ HD 7870 Processor: Intel® iCore™ i5-2500K or AMD® Phenom™ II X6 1090T Requires a 64-bit processor and operating system Steam account is required for game activation and installation. Surviving the Aftermath: Ultimate Colony Edition Learn more about the Surviving the Aftermath: Ultimate Colony Edition Model Name Uncover secrets surrounding the apocalypse and prepare your colony for survival if it should happen again. Discover the Truth: Specialists will stumble upon mysteries while exploring the world.The colony’s fate depends on your judgement, every choice can impact your colony’s happiness and affect its future. Make Tough Choices: Surviving the post-apocalypse means making impossible decisions.Specialists can set up outposts and gather materials, giving your colony an edge. Meet rival colony leaders and trade resources or compete for survival. Explore a Procedurally Generated World: Send your specialists outside your colony and into a procedurally generated world.Outfit your specialists with gear to defend the colony from attacking bandits and rampaging wildlife. Recruit and Manage Specialists: Recruit over 80 unique specialists, each with their own skills and motivations, to manage your colony’s resources.Stay prepared, a natural disaster or environmental catastrophe can doom an ill-equipped settlement. Construct more than 130 buildings and survive a harsh world filled with danger. Build and Manage a Colony: Build and manage a colony of survivors after a world-ending event.Go beyond the colony and explore the wastelands to gather resources, meet rival colonies, and uncover the world’s secrets. Survive and thrive in a post-apocalyptic future - resources are scarce, but opportunity calls! Build the ultimate disaster proof colony, protect your colonists, and restore civilization to a devastated world. The Ultimate Colony Edition includes Surviving the Aftermath, the Expansion Pass, and exclusive in-game content. Get everything you need to build a thriving colony of survivors with Surviving the Aftermath: Ultimate Colony Edition. You can never be too prepared when it comes to surviving the post-apocalypse.
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